Fusarium graminearum （F. graminearum）is the main pathogenic fungus that infects wheat， corn， and other important food crops. Meanwhile， deoxynivalenol secreted by F. graminearum is hard to be removed from contaminated food， it seriously threats human and livestock health. The biosynthesis pathway and molecular regulation mechanisms of DON production in F. graminearum have long been global research priorities. This knowledge is also important for the safety control of DON mycotoxin. The genes， ecoding protein and biological functions of the TRI-cluster genes cluster involved in the biosynthesis of DON in F. graminearum are summarized. The latest research progress on the regulatory mechanism of DON biosynthesis in F. graminearum is outlined. This information will be a valuable reference data for studies addressing the prevention and management of mycotoxin pollution on relevant food crops.