Objective To provide evidence for timely control measures， the pathogenic characteristics of Vibrio parahemolyticus isolated from a cluster diarrhea event in Nanxun District， Huzhou City， in 2021 were analyzed.Methods Fifty-nine suspected cases were identified by interviewing patients and checking hospitals. Twenty-nine anal swab samples were tested for etiology from cooks， waiters， and patients， and 18 food leftovers. Serotyping， molecular typing， tlh/tdh/trh virulence gene detection， and drug sensitivity testing of Vibrio parahaemolyticus isolates were performed by serological identification， pulsed field gel electrophoresis （PFGE）， multiplex fluorescent polymerase chain reaction， and micro-broth dilution methods， respectively.Results Vibrio parahaemolyticus was isolated from anal swab samples of 3 cooks and 6 cases， and the detection rate was 15.79%. The serotype of the isolates was O10：K4. All strains were positive for tlh and tdh and negative for trh. Nine isolates were only resistant to ampicillin and sensitive to the other 12 tested antibiotics. PFGE clustering analysis revealed a 100% similarity coefficient between isolates. The band type of isolates from cooks and patients was consistent.Conclusion The aggregation diarrhea was caused by Vibrio parahaemolyticus O10：K4. All isolates harbored the tdh virulence gene. Homology among the isolates was high.