Objective To provide reference for guiding consumption and carrying out safety supervision， the exposure of chloramphenicol （CAP） residues in commercially available animal-origin foods collected from Shenzhen local trading markets and supermarkets were assessed.Methods Five hundred and eleven samples were collected in Shenzhen City from 2018 to 2019. The CAP residues was determined by ultra-high performance liquid chromatography-tandem mass spectrometry， and dietary exposure was estimated by point assessment method. Exposure risk for different populations was characterized by the margin of exposure （MOE） values.Results No chloramphenicol was detected in 160 livestock and 60 poultry meat samples， and the detection rate of CAP was 26.80% in 291 aquatic samples， including shellfish， shrimp and fish. The CAP concentration in shellfish was in the range of 0.05-205.00 μg/kg， the mean concentration was 13.71 μg/kg， and the detection rate was 37.21% （64/172）. The CAP concentration in shrimp was in the range of 0.05-2.20 μg/kg， the mean concentration was 0.20 μg/kg， and the detection rate was 13.64% （64/172）. The CAP concentration in fish was in the range of 0.05-1.90 μg/kg， the mean concentration was 0.11 μg/kg， and the detection rate was 11.34% （11/97）. The detection rate of CAP in all animal-origin samples was 15.26% （78/511）. The result of dietary exposure assessment showed that the Chronic Daily Intake （CDI） values for average population for Shenzhen residents was 2.06×10^-3 μg/kg·BW， and the MOE value was 485； the CDI value for 5th percentile was 4.85×10^-5 μg/kg·BW， and the MOE value for was above 10 000； the CDI values for 50^th and 95^th percentile were 6.72×10^-4 μg/kg·BW and 9.25×10^-3 μg/kg·BW， respectively， and the MOE values were both below 10 000， suggesting some health risks.Conclusion Excessive consumption of animal-origin foods might lead to some health risk. In order to reduce the exposure risk， the population with higher exposure should limit their consumption， especially aquatic products. Meanwhile， the relevant departments should strengthen supervision and management of animal-origin foods quality and safety， and pay close attention to this problem.