Objective To establish a rapid qualitative and quantitative method for the determination of 8 bufo toxins in toad skin residual from poisoned patients by ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry （UPLC/Q-TOF-MS）.Methods After the toad skin samples were pretreated， the sample solution was separated on A Cquity UPLC^? HSS T3 column （100 mm×2.1 mm， 1.8 μm） with 0.1% formic acid solution as mobile phase A and methanol as mobile phase B. The flow rate was 0.3 mL/min， the column temperature was 35 ℃， and the sample volume was 2 μL.Results The linear ranges were 10.0-1 000.0 μg/L with r²≥0.990. The limits of detection were 0.7-2.1 μg/kg， and the limits of quantification were 2.5-7.0 μg/kg. The average recoveries were ≥75.79% and RSDs were ≤9.84%. The contents of bufalin， desacetylcinobufagin， desacetylcinobufotalin， pseudobufarenogin and bufotaline were 123.8±3.1， 5 920.4±1.9， 680.7±3.2， 671.6±9.1 and 1 796.6±74.9 μg/kg， respectively， while gamabufotain， cinobufagin and cinobufotalin were not detected.Conclusion The method is practical， rapid and reliable for the determination of cinobufagin， bufalin， pseudobufarenogin， desacetylcinobufotalin and desacetylcinobufagin， bufotaline， gamabufotain and cinobufotalin in toad skin.