Objective To analyze the pathogenic characteristics of Salmonella senftenberg isolate causing food poisoning in Xinxiang in 2021.Methods Epidemiological investigation of food poisoning event was performed. Pathogenic bacteria were separated and identified from 11 samples. Ten Salmonella isolates were subjected to serotyping， drug susceptibility testing， detection of characteristic gene fragments （mogA， sseL， mgtC， bcfA， araB） of 5 virulence island （SPIs） and pulsed field gel electrophoresis （PFGE） typing analysis.Results Serum antigenic formulas of all Salmonella isolates were 1，3，19；g，s，t Salmonella senftenberg. All Salmonella isolates were resistant to cefazolin， kanamycin， gentamicin and amikacin. Among them， two isolates from the patient feces were also resistant to ampicillin， tetracycline，doxycycline，chloramphenicol，trimethoprim/sulfamethoxazole. PFGE clustering analysis showed that there was no difference in the bands among the 10 strains （2 cases strains， 5 food strains， and 3 environmental strains）， suggesting that they were highly homologous. Five Salmonella pathogenicity island characteristic gene fragments were detected among 10 isolates.Conclusion Salmonella senftenberg caused the food poisoning event. The isolates carried 5 Salmonella pathogenicity island characteristic gene fragments and 2 isolates exhibited multi-drug resistance. Relevant departments should pay more attention to this issue.