Salmonella serotyping and molecular typing in meat products in Yibin city of Sichuan Province from 2015 to 2020
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1.College of Public Health, Chengdu Medical College, Sichuan Chengdu 610500, China;2.Yibin Center for Disease Control and Prevention, Sichuan Yibin 644600, China

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R155

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    Abstract:

    Objective To analyze the influencing factors of Salmonella contamination, the serotype distribution and molecular typing of Salmonella isolates in meat products in Yibin city were studied.Methods A total of 29 Salmonella strains isolated from commercial meat products in Yibin city of from 2015 to 2020 were analyzed by slide agglutination test for serotype and pulsed field gel electrophoresis(PFGE) for molecular subtyping. Multivariate Logistic regression analysis was carried out on the effects of geographical distribution, sample source and sales mode on the detection composition of Salmonella serogroup.Results Fourteen serotypes and 4 serogroups were detected in the Salmonella isolates. The dominant bacteria were Salmonella typhimuriumSalmonella give and Salmonella enteritidis. The PFGE cluster analysis showed 18 genotype. The genotypes of Salmonella enteritidis were highly homologous with similarity index of 100%, indicating the same source. Salmonella typhimurium with 4 genotypes and Salmonella give with 2 genotypes both had lower similarity. Multivariate Logistic analysis showed that the risk factor on serogroup D of Salmonella was geographical distribution (OR=1.053, P<0.05). The risk factors on serogroup E of Salmonella were sample source (OR=3.887, P<0.05) and geographical distribution (OR=1.227, P<0.05).Conclusion The poultry breeding sites and sample source are risk factors for Salmonella infection, and the hygienic inspection should be reinforced accordingly.

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LIU Xinling, WANG Xuan, DENG Kaiqi, HUANG Yue, ZHANG Ying, LI Jiuhong, MAO Li, ZHANG Hong.Salmonella serotyping and molecular typing in meat products in Yibin city of Sichuan Province from 2015 to 2020[J].中国食品卫生杂志,2023,35(3):321-325.

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History
  • Received:January 22,2022
  • Revised:
  • Adopted:
  • Online: May 24,2023
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