Determination of banned pigment quinoline yellow in pastries by salting out assisted-high performance liquid chromatography
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Shandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Shandong Ji’nan 250101, China

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R155

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    Abstract:

    Objective To develop an analytical method for fast determination of banned pigment quinoline yellow in pastries by salting out assisted-high performance liquid chromatography.Methods The sample was extracted with 40% methanol-sodium chloride-water, precipitated with potassium ferrocyanide-zinc acetate solution, eluted with mobile phase of methanol-0.02 mol/L ammonium acetate solution, separated by X-Bridge C18 column v(150 mm×4.6 mm, 3.5 μm), and detected with diode -array detector by external standard method.Results The method showed good linearity (r>0.999) in the range of 0.4-40.0 μg/mL. The limit of detection (S/N=3) was 1.25 mg/kg and the limit of quantification (S/N=10) was 5.0 mg/kg. The average recoveries of three different concentrations level at 5.0, 10.0 and 50.0 mg/kg ranged from 89.18% to 110.10%, with relative standard deviation in the range of 2.83%-8.65%.Conclusion The method was convenient, accurate and reproducible, and it was suitable for qualitative and quantitative analysis of banned pigment quinoline yellow in pastries.

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SUN Shanshan, WANG Mingdong, ZHAO Huinan, ZHENG Wenjing, XUE Xia, HU Mei, WANG Jun, LIU Yanming, ZHANG Yanxia. Determination of banned pigment quinoline yellow in pastries by salting out assisted-high performance liquid chromatography[J].中国食品卫生杂志,2023,35(2):198-203.

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History
  • Received:December 30,2021
  • Revised:
  • Adopted:
  • Online: April 18,2023
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