Objective The detection method of rapid analysis of chlorpyrifos and its degradation products in vegetables and fruits by HPLC-MS/MS was established.Methods Both chromatographic conditions and mass spectrometry conditions were optimized. Leek， celery， cabbage， apple and citrus were used as substrates for pretreatment by QuEChERS method， and the effects of PSA and GCB on chlorpyrifos and its degradation products were investigated. Finally， the method was investigated based on limits of detection， limits of quantification， linear range， matrix effect， accuracy and precision.Results The suitable mobile phase was acetonitrile and 0.1% （V/V） formic acid in water. Acquisition parameter of mass spectrometer was ESI⁺. 45 mg/5 mL of GCB in pretreatment was the best purification condition. The linearity of the four compounds was good （R²≥0.99）， and the limits of quantification （LQD） were 1-30 μg/kg. The average recoveries of three levels ranged from 66.1% to 113.6%， and the relative standard deviation （RSD） was 1.3%-16.9%.Conclusion The method is fast， efficient， accurate， repeatable and suitable for the confirmation and quantification of chlorpyrifos and its degradation products in vegetables and fruits.