Objective To evaluate the physical fatigue relief function of ginseng compound formula beverage （ginseng compound） and explore its molecular mechanism.Methods Two hundred and fifty healthy male KM mice were divided into 5 groups with 50 mice in each group， including control group， sugar control group and 10 times concentrated beverage of ginseng compound groups： 4.16， 8.33 and 16.66 mL/kg·BW. After continuously gavage for 30 d， 20 mice for weight-bearing swimming test， and the remaining 30 mice in each group were randomly selected for blood lactic acid， liver glycogen and exhaustive swimming experiments， based on the exhaustion experiment （high-urea model） to carry out mechanism exploration， including the effects of test subjects on exhaustion exercise-induced injury， antioxidant pathway and mitochondrial autophagy pathway of quadriceps femoris muscle.Results Compared with the control group， mice in the ginseng compound group were more active， and the weight-bearing swimming time of mice in the 4.16 and 16.66 mL/kg·BW groups increased significantly （P<0.05）. The area integral of blood lactic acid decreased significantly in the three dose groups （P<0.05）. Blood urea nitrogen of 4.16 mL/kg·BW group in the exhaustion experiment decreased significantly （P<0.05）， Nrf2 transcript level in skeletal muscle， activities of superoxide dismutase and catalase increased significantly （P<0.05）， PINK1-Parkin transcript level decreased significantly （P<0.05）. Creatine kinase， lactate dehydrogenase and malonaldehyde in serum decreased significantly in the three dose groups （P<0.05）.Conclusion Ginseng compound has the effect of relieving physical fatigue， and its mechanism may be related to the reduction of skeletal muscle mitophagy caused by exhaustion through the Nrf2/ARE-PINK1-Parkin pathway.