Objective To investigate two food poisoning incidents in Quzhou City， determine the causes of the incidents， and provide basis and suggestions for the prevention and control of similar poisoning incidents.Methods Epidemiological investigation was carried out on two food poisoning incidents in Quzhou City in 2020 and 2021， and the causes of the incidents was determined by comprehensive analysis of food exposure history， clinical symptoms and characteristics， laboratory test results， etc.Results A total of 14 cases were diagnosed in these two food poisoning incidents， and the incubation period was 20 min to 4 h. The main clinical symptoms of 14 cases were similar， including diarrhea， abdominal pain， nausea， vomiting and dizziness. All cases had a history of eating bitter gourd. In these two food poisoning incidents， cucurbitacin B were detected by ultra performance liquid chromatography-triple quadrupole mass spectrometry using atmospheric pressure chemical ionization. The content of cucurbitacin B in bitter gourd sample was 150 mg/kg， the content of cucurbitacin B in leftover sample containing bitter gourd was 69.9 mg/kg. The content of cucurbitacin B in blood sample of one case was 2.7 μg/L， and the content of cucurbitacin B in 5 urine samples was 0.14-0.59 μg/L.Conclusion These two food poisoning incidents were caused by bitter lagenaria siceria. It is suggested that the food regulatory authority and the health department should strengthen health education among caterting service personnel and the public to prevent the poisoning incidents.