Investigation and molecular typing of Staphylococcus aureus in cold dishes sold in the market of Ya’an
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1.Sichuan Agricultural University Food College, Institute of Food Processing and Safety , Sichuan Ya’an 625014, China;2.Sichuan Food Inspection ReseArch Institute, Sichuan Chengdu 611731, China

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R155

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    Abstract:

    Objective To investigate the contamination status, virulence gene distribution, biofilm formation ability and molecular typing characteristics of Staphylococcus aureus S.aureus in some commercial cold dishes.Methods Two hundred cold dishes sold in restaurants, supermarkets and street vendor stalls were sampled, and Staphylococcus aureusS.aureus were separated. Congo red plate method and crystal violet staining method were used to detect the biofilmforming ability of the isolates. Virulence genes and biofilm genes were detected by PCR, as well as agr typing and multil-ocus sequence typing (MLST).Results Twenty four strains of S.aureus were isolated, and the detection rate was 10.50%(21/200); the detection rate of cold dishes was 13.73%(14/102), and the detection rate of stewed meat product was 7.14%(7/98); the highest detection rate of seA in the traditional enterotoxin gene was 50.00%(12/24), and the lowest of seE was 16.67%(4/24). Poisoning shock syndrome toxin-1 (TSST-1) and leukotoxin (PVL) were 4.17%(1/24) and 25.00%(6/24), respectively. The detection rate of hemolysin gene was relatively high, and only etd was detected in the dermatoxin gene. The positive rate was 62.5%(15/24) in Congo red plate test and 100% in crystal violet staining; the biofilm genes were mainly cna and clfB, and bap was not detected;the molecular typing of the strains was mainly agr Ⅰ and ST188.Conclusion The S.aureus contamination exsits in some cold dishes sold in Ya’an city. The strains carried multiple virulence genes, had strong film-forming ability and had potential food safety risks.

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WU Renzhi, ZHANG Yi, WANG Xin, DAI Mingtao, HE Xiaorong, HE Qiaoling, HAN Guoquan. Investigation and molecular typing of Staphylococcus aureus in cold dishes sold in the market of Ya’an[J].中国食品卫生杂志,2022,34(6):1257-1262.

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History
  • Received:September 28,2021
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  • Online: February 06,2023
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