Determination of 11 N-nitrosamines in Chinese pickles and salted vegetables by QuEChERS combined with GC-MS/MS
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Xianju County Food and Drug Inspection and Testing Center, Zhejiang Taizhou 317300, China

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R155

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    Abstract:

    Objective To establish an efficient analytical method for simultaneous determination of 11 N-nitrosamines in Chinese pickles and salted vegetables by QuEChERS combined with gas chromatography-mass spectrometry.Methods The sample was extracted with acetonitrile, dehydrated by 4 g anhydrous magnesium sulfate and 1 g sodium chloride, purified with 150 mg PSA, 150 mg GCB and 900 mg anhydrous magnesium sulfate, separated by HP-Innowax chromatographic column, and quantitatively analyzed in the multi-reaction monitoring mode. The matrix effect was evaluated by calculating the ratio of the slope between the matrix and the solvent standard curve.Results In the range of 0.5-50 μg/L, the linear coefficient of 11 N-nitrosamines were all above 0.999. NDBA, NMPhA, NEPhA and NDPhA showed stronger matrix effects, and the standard solution should be prepared by the blank matrix solution. The average recovery rate of low, medium and high levels (1, 3, 10 μg/kg) in the sample spiking with reference standard was 94.8%-105.1%, of which the RSD (n=6) was 1.6%-5.8%.Conclusion With simple pretreatment operation, good reproducibility and accurate results, this method is suitable for the quantitative analysis of 11 N-nitrosamines in Chinese pickles and salted vegetables.

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GUAN Yue, PAN Yanmei. Determination of 11 N-nitrosamines in Chinese pickles and salted vegetables by QuEChERS combined with GC-MS/MS[J].中国食品卫生杂志,2022,34(6):1226-1232.

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  • Received:May 12,2021
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  • Online: February 06,2023
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