Objective To provide reliable technical support for the prevention and control of foodborne diseases and improve the ability to deal with food poisoning emergencies， the epidemic characteristics of Vibrio parahaemolyticus（VP）in Liaoning Province was studied.Methods The PFGE molecular typing， serological typing and drug sensitivity test of VP isolated from patients and food in 14 cities of Liaoning Province in 2020 were carried out and analyzed.Results Among the 44 strains of VP （22 isolates from foodborne diseases and 22 isolates from food）， 8 strains could not be identified with K-type， and the untyping rate was 18.2%. The other strains were divided into 15 serotypes and 9 serogroups. The isolates from foodborne diseases were divided into 4 serotypes and 3 serogroups. The main serogroup was O3， followed by O1. Foodborne isolates were divided into 11 serotypes and 6 serogroups. The main serogroup was O2， followed by O1 and O3. The results of drug sensitivity of 44 strains of VP to 15 antibiotics showed that the resistance rate of cefazolin was 68.2%， followed by ampicillin（22.7%）， erythromycin（13.6%）， azithromycin（4.5%）， cefoxitin（2.3%） and cefotaxime（2.3%）. The main strains were resistant to β-lactamand macrolide antibiotics. Drug resistance ability increased year by year， some strains showed multiple drug resistance， and the number increased year by year. The drug resistance rate of foodborne disease isolates was higher than that of food isolates. The drug resistance rate of the main epidemic strains with serotype O3：K6 was high. PFGE molecular typing could be divided into five PFGE dominant bands according to the similarity of more than 90%. VP isolated from the patients had high similarity and could be clustered into 3 groups. The similarity of VP isolated from food was low. Except that the similarity of two strains was greater than 90%， the similarity between other strains was less than 85%.Conclusion There was a high homology between O3 and O1 serotype strains， suggesting that O3 and O1 serotype strains were closely related. The homology between food strains was low， suggesting low correlation. The drug resistance of VP isolates in Liaoning Province in 2020 was not optimistic， and the drug resistance trend needed further attention.