Objective After polymyxin was banned as growth promotion in animals， the carrying status of mcr-1 gene of Escherichia coli（E.coli） in chickens in some areas of Sichuan Province was investigated to provide basis for further prevention and control measures.Methods Rectal swabs were collected from broilers in some markets in Sichuan Province. The bacteria were inoculated with E.coli broth containing polymyxin （final concentration 4 μg/mL） and macconkey plate containing polymyxin （final concentration 4 μg/mL）. Suspicious colonies were selected and identified by PCR and mcr-1 gene was detected. Microbroth dilution method was used to determine the resistance of mcr-1 gene positive strains to common antibiotics. Pulse field gel electrophoresis （PFGE） was used to analyze the homology of mcr-1 gene positive strains. mcr-1 gene transmission route was verified by plasmid binding assay.Results mcr-1 positive Escherichia coli was detected in 13 samples of 70 broiler chickens， with a detection rate of 18.57% （13/70）. Among the 13 antibiotics tested， 13 mcr-1 positive strains showed different degrees of drug resistance to antibiotics， except that 12 strains were sensitive to cefoxitin. The drug resistance rates of tetracycline and trimethoprim/sulfamethoxazole were the highest， reaching 100% （13/13）. Ampicillin and chloramphenicol followed with resistance rate of 84.62% （11/13）. PFGE showed that 13 mcr-1 positive Escherichia coli strains belonged to 13 different types. Plasmid binding experiments showed that mcr-1 gene could be transmitted through plasmids.Conclusion The detection rate of mcr-1 gene in chicken E.coli is high， and the drug resistance of mcr-1 positive E.coli in chicken is serious.