Objective To investigate the microbial contamination of liquid milk in some municipalities in Hubei Province in 2021.Methods A total of 101 raw milk， pasteurized milk and sterilized milk from production， wholesale and retail were collected. The total number of bacterial colonies， aerobic Bacillus and thermophilic aerobic Bacillus， alkaline phosphatase of the samples were detected.Results The overall exceeding rates of raw milk， pasteurized milk and sterilized milk （any one of the four indicators exceeded the standard） were 52.94%， 8.70% and 1.64%， respectively. The detection rates of aerobic Bacillus were 52.94%， 4.35% and 1.64%， respectively. The value of alkaline phosphatase in one retail whole fat pasteurized milk sample was 409 mU/L. The shelf days of pasteurized milk had no correlation with the value of alkaline phosphatase （r=0.10， P=0.65）. The detection rates of samples exceeding the standard in production and processing sector and circulation were 52.94% and 3.57%， respectively， with statistically significant differences （χ²=32.92， P<0.001）. There was no significant difference in the exceeding rate of samples between convenience stores/whole sale markets and shopping mall/supermarkets （P>0.05）.Conclusion The pollution of aerobic Bacillus in raw milk was common in Hubei Province. Pasteurized milk and sterilized milk had the problem of microbial contamination which violated the standard. It is recommended to further investigate the causes， analyze the sources of contamination， provide the basis for targeted measures， and strengthen the monitoring of the total number of aerobic Bacillus， alkaline phosphatase and other indicators in liquid milk， which can provid baseline data for establishing appropriate limit standards.