Objective A qualitative and quantitative method has been established to rapidly determine carnitine enantiomers in dietary supplement by ¹H-nuclear magnetic resonance combined with chiral lanthanide shift reagents （CLSR-¹H qNMR）.Methods Pyrazine acted as internal standard was added to the samples dissolved in d₄-methanol. After vortexing， ultrasonic-assisted extraction and centrifugation， an appropriate amount of supernatant and chiral lanthanide shift reagent （CLSR） Eu（thfc）₃ were added to the NMR tube for directly determination.Results The linearity range of L-carnitine was 16.3～4 800 μg/mL with correlation coefficient （r）>0.99. The limit of detection （LOD） was 24.5 mg/kg， and the limit of quantification （LOQ） was 73.5 mg/kg. While the linearity range of D-carnitine was 10.6～530 μg/mL with r>0.99. The LOD was 27.5 mg/kg， and the LOQ was 82.5 mg/kg. Recovery rates obtained from spiked samples were between 95.9% and 114.6% with the RSDs （n=6） of 3.31%~6.12%. Tests on capsules， milk tea， coffee powder and tablet from online shopping that claimed to have weight-loss effects failed to detect D-carnitine.Conclusion This method is simple， rapid and sensitive. And the sample test could be completed within 25 min， which could provide a theoretical basis for rapidly detection of carnitine enantiomers.