Objective A high-throughput method for simultaneous determination of 67 herbicide residues in oilseeds and oils by liquid chromatography-tandem mass spectrometry combined with dispersive solid phase extraction was established.Methods Peanut， soybean， rapeseed and their oil were extracted with 1% formic acid-acetonitrile， and then purified by ethylenediamine-N-propyl and anhydrous MgSO₄ dispersed solid phase extraction. Methanol and 5 mmol/L ammonium acetate （containing 0.1% formic acid） were used as mobile phases for gradient elution. C₁₈ column was used for separation， positive and negative ion mode of ESI ion source was used for simultaneous multi-reaction monitoring （MRM）. And matrix standard curve external standard method was used for quantification.Results Under different substrates， the linear range of 67 herbicides was from 0.000 5 to 0.08 mg/L， the correlation coefficients （R²） were all above 0.992， and the limits of detection （LODs） were 0.005~0.02 mg/kg. The recoveries of three spiked levels （LOD， 2LOD and 10LOD） were between 62.3% and 118.1% with RSD （n=6） less than 15%.Conclusion This method is stable， specific， sensitive and accurate， and can be used for qualitative and quantitative detection of herbicides in oilseeds and oils.