Rapid screening of carbendazim residues in fruits and vegetables using surface enhanced Raman spectroscopy based on the model of probability of detection
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1.Technical Center for Animal Plant and Food Inspection and Quarantine, Shanghai Customs, Shanghai 200135, China;2.Shanghai Oceanhood Opto-eletronics Tech Co., LTD, Shanghai 201209, China

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R155

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    Abstract:

    Objective To develop a detection method for rapid screening of carbendazim residues in fruits and vegetables using surface enhanced Raman spectroscopy (SERS) based on the model of probability of detection.Methods The test solution was obtained after ethanol extraction, purification using dichloromethane, nitrogen blowing concentration and ethanol re-dissolution. The parameters including the addition amount of gold nanoparticles, extraction solution, extraction reagent and coagulant were optimized by single factor experiment, and the instrument test conditions of Raman spectrometer were determined.Results The displacement of carbendazim was 630±5 cm-1, 728±5 cm-1, 1,002±5 cm-1, 1,224±5 cm-1, 1,264±5 cm-1 and 1,316±5 cm-1. According to its Raman spectrum characteristic peak and its intensity, carbendazim was determined qualitatively and rapidly. With the probability of detection (POD), the detection limit of carbendazim in apple, citrus and other fruits was 0.5 mg/kg, and that in pepper and other vegetables was 1.0 mg/kg.Conclusion This method is simple, fast and accurate. It takes only 40 min from sample pretreatment to the results. Combined with POD model, the effectiveness of the method is verified. And the method can be used for field rapid detection of carbendazim residues in fruits and vegetables.

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CHEN Niannian, GU Shuqing, REN Shuo, ZHAO Chaomin, DENG Xiaojun, ZHAO Zhihui. Rapid screening of carbendazim residues in fruits and vegetables using surface enhanced Raman spectroscopy based on the model of probability of detection[J].中国食品卫生杂志,2022,34(4):700-706.

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History
  • Received:December 31,2021
  • Revised:
  • Adopted:
  • Online: August 26,2022
  • Published: