Home > Archive>Volume 34, Issue 3, 2022 >504-509. DOI:10.13590/j.cjfh.2022.03.017
PDF HTML XML Export Cite reminder
Effect of antimicrobial addition in pre-enrichment broth for Shiga toxin-producing Escherichia coli isolation
CSTR:
[cstr]
Author:
Affiliation:

1.College of Public Health, Zunyi Medical University, Guizhou Zunyi 543006, China;2.National Institutes for Food and Drug Control, Beijing 100025, China;3.Key Laboratory of Food Safety Risk Assessment, National Health Commission of the People’s Republic of China, China National Center for Food Safety Risk Assessment, Beijing 100025, China

Clc Number:

R155

  • Article
    • | |
  • Metrics
    • |
  • Reference [30]
    • | | | |
  • Comments
    Abstract:

    Objective To study the effects of the kind and concentration of antibiotics in pre-enrichment for the detection of Shiga toxin-producing Escherichia coli （STEC） in the current international standard method.Methods The minimal inhibitory concentrations （MICs） of three antimicrobials supplemented in an STEC enrichment broth of four official methods were measured through testing of a panel of STEC and non-STEC isolates.Results There were differences among different antibiotics for the inhibition of STEC. At the recommended concentration， acriflavine and cefsulodin inhibited the growth of stx1a and stx2b subtypes of STEC， while novobiocin inhibited the growth of stx1a， stx1c， stx1d， stx2b， stx2d， stx2e， stx2f and stx2g subtypes of STEC. In addition， there was no significant difference among MICs of STEC and other gram-negative bacteria to acriflavine， cefsulodin and novobiocin （P>0.05）. MICs of gram-positive strains to three tested antimicrobials were significantly lower than those of gram-negative bacteria （P<0.01）.Conclusion The data provided valuable evidence for the STEC enrichment method development and improvement.

    Reference
    [1] ZUMBRUN S D， MELTON-CELSA A R， SMITH M A， et al. Dietary choice affects Shiga toxin-producing Escherichia coli （STEC） O157：H7 colonization and disease[J]. PNAS， 2013， 110（23）： E2126-E2133.
    [2] MAJOWICZ S E， SCALLAN E， JONES-BITTON A， et al. Global incidence of human Shiga toxin-producing Escherichia coli infections and deaths： A systematic review and knowledge synthesis[J]. Foodborne Pathogens and Disease， 2014， 11（6）： 447-455.
    [3] COINTE A， BIZOT E， DELANNOY S， et al. Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe. Toxins （Basel）， 2021，26；13（10）：686.
    [4] DE RAUW K， JACOBS S， PIéRARD D. Twenty-seven years of screening for Shiga toxin-producing Escherichia coli in a university hospital. Brussels， Belgium， 1987-2014[J]. PLoS One， 2018， 13（7）： e0199968.
    [5] SCHEUTZ F， TEEL L D， BEUTIN L， et al. Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature[J]. Journal of Clinical Microbiology， 2012， 50（9）： 2951-2963.
    [6] Centers for Disease Control and Prevention （CDC）. National enteric disease surveillance： Shiga toxin-producing Escherichia coli （STEC） annual report， 2014[R/OL]. （2017-02-08）[2022-03-29]. https：//www.cdc.gov/nationalsurveillance/pdfs/STEC-2014-REPORT_508c.pdf.
    [7] US Department of Agriculture， Food Safety Inspection Service. Sampling verification activities for Shiga toxin-producing Escherichia coli （STEC） in raw beef products - Revision 4 [EB/OL]. WASHINGTON， DC： FSIS Directive 10，010.1， 2015.
    [8] 厚生省医药食品局安全对策科. 肠道出血性大肠杆菌关于O26、O103、O111、O121、O145及O157的检查法： 食安监发1120第1号 [S]. 东京： 日本厚生劳动省， 2014.
    [9] International Organization for Standardization （ISO）. Microbiology of food and animal feed—real-time polymerase chain reaction （PCR）-based method for the detection of food-borne pathogens—horizontal method for the detection of Shiga toxin-producing Escherichia coli （STEC） and the determination of O157， O111， O26， O103 and O145 serogroups： ISO/TS 13136：2012 （en）[S]. Geneva， Switzerland： International Organization for Standardization， 2012.
    [10] FENG P， WEAGANT S D， KAREN J. BAM Chapter 4A： Diarrheagenic Escherichia Coli[M]. United States Food and Drug Administration： Silver Spring， MD， USA， 2020.
    [11] The Federal Meat Inspection Act. 21 Code chapterU.S. 12-Meat inspection[EB/OL]. （2019-12-20）[2022-03-29]. https：//www.law.cornell.edu/uscode/text/21/chapter-12.
    [12] Official Journal of the European Union. Commission Regulation （EU） No 209/2013 of 11 March 2013 amending regulation （EC） No 2073/2005 as regards microbiological criteria for sprouts and the sampling rules for poultry carcases and fresh poultry meat[S/OL]. Official Journal of the European Union， 2013. https：//www.fsai.ie/uploadedFiles/Reg209_2013.pdf.
    [13] BENNETT S D， SODHA S V， AYERS T L， et al. Produce-associated foodborne disease outbreaks， USA， 1998-2013[J]. Epidemiology and Infection， 2018， 146（11）： 1397-1406.
    [14] FSANZ. Compendium of microbiological criteria for food[S/OL]. Australia and New Zealand： Food Standards Australia New Zealand， 2018. https：//www.foodstandards.gov.au/publications/Documents/Compedium%20of%20Microbiological%20Criteria/Compendium_revised-Sep%202018.pdf.
    [15] AFDS. Food code： NO.2021-54[S/OL]. Chungcheongbuk-do， Republic of Korea： Administration of Food and Drug Safety ， 2021. https：//www.mfds.go.kr/eng/brd/m_15/view.do？seq=69982.
    [16] 食品安全中心. 即食食品微生物指南[Z/OL]. 香港特别行政区： 食品环境卫生署， 2002.
    [17] 国家卫生和计划生育委员会. 食品安全国家标准 预包装食品中致病菌限量： GB 29921—2021[S]. 北京： 中国标准出版社， 2021.
    [18] WASILENKO J L， FRATAMICO P M， NARANG N， et al. Influence of primer sequences and DNA extraction method on detection of non-O157 Shiga toxin-producing Escherichia coli in ground beef by real-time PCR targeting the eae， stx， and serogroup-specific genes[J]. Journal of Food Protection， 2012， 75（11）： 1939-1950.
    [19] WAYNE P. Clinical and Laboratory Standards Institute： Performance standards for antimicrobial susceptibility testing： 20th informational supplement[J]. CLSI document M100-S20， 2010.
    [20] NAKAMURA K， OGURA Y， GOTOH Y， et al. Prophages integrating into prophages： A mechanism to accumulate type III secretion effector genes and duplicate Shiga toxin-encoding prophages in Escherichia coli. PLoS Pathog， 2021，17（4）：e1009073.
    [21] KRüGER A， LUCCHESI P M A. Shiga toxins and stx phages： Highly diverse entities[J]. Microbiology： Reading， England， 2015， 161（P3）： 451-462.
    [22] AMAGLIANI G， ROTUNDO L， CARLONI E， et al. Detection of Shiga toxin-producing Escherichia coli （STEC） in ground beef and bean sprouts： Evaluation of culture enrichment conditions[J]. Food Research International， 2018， 103： 398-405.
    [23] CUI S， MENG J， BHAGWAT A A. Availability of glutamate and arginine during acid challenge determines cell density-dependent survival phenotype of Escherichia coli strains[J]. Applied and Environmental Microbiology， 2001， 67（10）： 4914-4918.
    [24] GILL A， CARRILLO C， HADLEY M， et al. Bacteriological analysis of wheat flour associated with an outbreak of Shiga toxin-producing Escherichia coli O121[J]. Food Microbiology， 2019， 82： 474-481.
    [25] KHAN S B， ZOU G， XIAO R， et al. Prevalence， quantification and isolation of pathogenic Shiga toxin Escherichia coli O157：H7 along the production and supply chain of pork around Hubei Province of China[J]. Microbial Pathogenesis， 2018， 115： 93-99.
    [26] ZELYAS N， POON A， PATTERSON-FORTIN L， et al. Assessment of commercial chromogenic solid media for the detection of non-O157 Shiga toxin-producing Escherichia coli （STEC）. Diagn Microbiol Infect Dis， 2016， 85（3）：302-308
    [27] STROMBERG ZR， LEWIS GL， MARX DB， et al. Comparison of Enrichment Broths for Supporting Growth of Shiga Toxin-Producing Escherichia coli. Curr Microbiol. 2015，71（2）：214-9.
    [28] Lázár V， SINGH G PAL， SPOHN R， et al. Bacterial evolution of antibiotic hypersensitivity. Mol Syst Biol， 2013，9：700.
    [29] AMAGLIANI G， ROTUNDO L， CARLONI E， et al. Detection of Shiga toxin-producing Escherichia coli （STEC） in ground beef and bean sprouts： evaluation of culture enrichment conditions[J]. Food Research International， 2018， 103： 398-405.
    [30] FRATAMICO P M， BHAGWAT A A， INJAIAN L， et al. Characterization of Shiga toxin-producing Escherichia coli strains isolated from swine feces[J]. Foodborne Pathogens and Disease， 2008， 5（6）： 827-838.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

HU Ying, ZHAO Linna, BAI Li, CUI Shenghui. Effect of antimicrobial addition in pre-enrichment broth for Shiga toxin-producing Escherichia coli isolation[J].中国食品卫生杂志,2022,34(3):504-509.

Copy
Share
Article Metrics
  • Abstract:409
  • PDF: 3145
  • HTML: 377
  • Cited by: 0
History
  • Received:March 29,2022
  • Online: July 07,2022
Article QR Code
您是第1764257 位访问者
网站版权所有：《中国食品卫生杂志》编辑部
技术支持：北京勤云科技发展有限公司

京公网安备 11010802028445号