Study on the quality control standard of culture medium for Salmonella
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National Institutes for Food and Drug Control, Beijing 100050, China

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R155

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    Abstract:

    Objective The purpose of this study was to provide basis for the revision of GB 4789.28—2013 and the quality control of culture media by screening the quality control strains of culture media for salmonella test.Methods The strains specified in GB 4789.28 and 9 salmonella strains(Salmonella newport, salmonclla Infantis, Salmonella choleraesuis, Salmonella blegdam, Salmonella kottbus, Salmonella arizona, Salmonella dublin, Salmonella agona, Salmonella. anatis) isolated from real food were used to test the growth rate and selectivity of 10 different brands of media including Rappaport Vassiliadis Salmonella Enrichment, Selenite Cystine Broth, Tetrathionate Broth Base enriched broth, Bismuth Sulfite Agar, Hektoen Enteric Agar, Xylose Lysine Desoxycholate Agar by quantitative and semi-quantitative methods. It was evaluated whether the quality control strain used in GB4789.28—2013 could meet the quality control of salmonella media.Results The results showed that the growth rate and selectivity of different media brands were different when the same strain was used, and Salmonella choleraesuis and Salmonella arizonae had higher requirements on the quality of media.Conclusion It is suggested that medium production enterprises and microbiology laboratories should use strains isolated from food with higher quality requirement in medium testing, and the quality control methods should be highly standardized to ensure the stability and reliability of test data.

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CHEN Yiwen, ZHANG Xiaodong, YU Wen, REN Xiu, LIU Na, CUI Shenghui. Study on the quality control standard of culture medium for Salmonella[J].中国食品卫生杂志,2022,34(3):491-497.

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History
  • Received:December 06,2021
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  • Online: July 07,2022
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