Objective To investigate the molecular types and antimicrobial resistance of Salmonella isolated from foodborne disease surveillance in Liaoning Province during 2016—2020 and provide reliable scientific basis for the prevention and control of Salmonella infection and antibiotic treatment.Methods A total of 90 strains of Salmonella isolated from clinical diarrhea cases in foodborne disease surveillance in Liaoning Province from 2016 to 2020 were detected for drug sensitivity by broth dilution method. The molecular typing was analysed by pulsed-field gel electrophoresis （PFGE） method. Bionumeries 7.6 software was used to analyze the similarity between strains.Results The 90 Salmonella strains were divided into 13 serotypes， of which Salmonella typhimurium and Salmonella enteritidis were dominant. The 90 strains were divided into 54 PFGE patterns by Xba I. The similarity between each pattern was 51.4%~100%， including 1~10 strains. The same serotype strains had highly similar PFGE pattern， and there was multiple clustering phenomenon. The drug susceptibility results showed that 90 strains of Salmonella showed 36 kinds of drug resistance spectrum.The resistance rate to ampicillin was the highest （66.7%）， followed by that to nalidixic acid （62.2%）. All strains were sensitive to cefoxitin.The overall multi-drug resistance rate reached 48.9% （44/90）， among which the predominant serotypes of Salmonella typhimurium and Salmonella enteritidis were 87.5% and 45.8%.Conclusion The cases of diarrhea caused by Salmonella in Liaoning Province are sporadic， but the trend of multi-drug resistance is obvious， the overall drug resistance rate is relatively high， and the drug resistance spectrum is wide. Traceability and drug resistance monitoring should be further strengthened. It is suggested that molecular tracing and drug resistance surveillance for Salmonella should be further strengthened.