Objective To establish a real-time polymerase chain reaction (PCR) rapid detection method for Pangasius bocourti-derived components. To establish a real-time PCR rapid detection method for Pangasius bocourti-derived components.Methods Primers were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and real-time PCR was used for amplification to achieve the purpose of rapid detection of products. Primers were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and real-time PCR was used for amplification to achieve the purpose of rapid detection of products. Results This method had good specificity, and the sensitivity could reach 10-4 ng Pangasius bocourti DNA. Pangasius bocourti cytb gene could be detected in fish products mixed with rice noodles, sesame powder, chicken powder and atlantic cod meal, and the sensitivity could reach 0.01%. This method had good specificity, and the sensitivity could reach 10-4 ng Pangasius bocourti DNA. Pangasius bocourti cytb gene could be detected in fish products mixed with rice noodles, sesame powder, chicken noodles and atlantic cod meal, and the sensitivity could reach 0.01%.Conclusion This method had high specificity, short time and high sensitivity, and could meet the detection requirements of Pangasius bocourti adulteration in fish meat products. This method had high specificity, short time and high sensitivity, and could meet the detection requirements of Pangasius bocourti adulteration in fish meat products.