Objective To study the toxic effects of Galerina sulciceps on mice. Methods The poisonous mushrooms collected from food poisoning were identified as Galerina sulciceps. The detected α-amanodexin peptide (α-AMA) was 1 690 μg/g. The mice were designed into 4 dose groups of 464,215, 100,46.4 mg/kg BW. After intraperitoneal injection, heart, liver, spleen, lung, kidney, brain, and intestine of mice were taken for pathological diagnosis. ResultsIn animal experiments, the median lethal dose(LD₅₀) of female mice was 108 mg/kg BW (confidence limit:74.1-157 mg/kg BW). The pathology of the liver of mice showed that with the increase of dose, hepatocyte swelling and necrosis, accompanied by bleeding and inflammatory cell infiltration, the pathological changes gradually worsened. Conclusion Galerina sulciceps mainly contains α-AMA, which causes liver damage to humans and animals. Studying the toxic mechanism of Galerina sulciceps has great significance to improve the survival rate of poisoned patients.