Objective To provide the basis scientific evidence for the timely response of this kind of poisoning incident by field epidemiological investigation and the identification of poisonous mushroom of two poisoning incidents in Shaowu, Fujian Province. Methods Case data, field epidemiological investigation data and suspected poisonous mushroom samples were collected for morphological and internal transcribed spacer molecular biological identification, and the event was analyzed and managed. Results On September 1 and 2,2018, two mushroom poisoning incidents occurred in Dafugang town and Xiaojiafang town, Shaowu City, 3 villagers ate wild mushrooms and suffered from nausea, vomiting, dizziness, excessive sweating, abdominal pain, diarrhea and other symptoms within 0.5 to 1.5 hours after meals. They were sent to the hospital for treatment and diagnosed as mushroom poisoning. Gastroenteritis and choline poisoning were the main poisoning manifestations. After admission, patients were given symptomatic support treatment and discharged from the hospital at 3-4 d. The sample of poisonous mushroom was identified as Entoloma omiense by morphology and molecular biology. Conclusion Two poisoning incidents were caused by eating Entoloma omiense by mistake. The research on this kind of mushroom should be strengthened to prevent the occurrence of this kind of poisoning.