Objective To understand the types and extent of mycotoxins in grains and its products sold in Henan Province. Methods During 2018-2019,16 kinds of mycotoxins were detected by isotope dilution ultra high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The results were evaluated and analyzed according to GB 2761-2017 national food safety standard mycotoxin limit in food. Results Fumonisin, zearalenone, aflatoxin and deoxynivalenol were the main mycotoxins in 100 corn flour, corn dregs and corn kernels, the detection rate ranged from 0.0% to 95.7%. The detection rates of deoxynivalenol in 220 wheat flour, noodles and steamed bread were 78.0%(124/159), 64.3%(18/28) and 87.9%(29/33) respectively, and the content of other mycotoxins was very low or not detected. Conclusion There were different levels of mycotoxin pollution in grain and its products in Henan Province, especially fumonisin in corn and its products. It is necessary to carry out traceability investigation in time and take corresponding control measures as soon as possible.