Objective To investigate the contamination, virulence-associated genes and molecular typing of Vibrio vulnificus and Vibrio fluvibrio in aquatic products of Guangzhou City. Methods Vibrio vulnificus and Vibrio fluvibrio suspected isolates were further identified by VITEK-2 system and polymerase chain reaction (PCR). The virulence-associated genes of the Vibrio isolates were determined, and genotyping analysis was performed by enterobacterial repetitive intergenic consensus-polymerase chain reaction (Eric-PCR). Results The positive rates were 10.4% (31/298) and 5.0% (15/298) for Vibrio vulnificus and Vibrio fluvibrio. Three genotypes of Vibrio vulnificus were detected, including CB, EA and CA. The virulence-associated genes vfh and toxR of Vibrio fluvibrio were all positive, the detection rate of hupO was 60.0% (9/15) and vfp was 80.0% (12/15). Eric-PCR amplified 8-14 bands between 100-2 000 bp, and 15 Vibrio fluvibrio strains were divided into 5 groups and 11 types with a similarity coefficient of 0.8. Conclusion The contamination of Vibrio vulnificus and Vibrio fluvibrio in aquatic products in Guangzhou was serious, and most virulence-associated genes were present. Eric-PCR result showed that 15 Vibrio vulnificus strains were related to each other in genetic relationship, so prevention and control should be strengthened.