Objective To establish a method for the simultaneous determination of 43 compounds of semi-volatile organic compounds (SVOCs) in drinking water by gas chromatography-mass spectrometry with automatic bulk solid phase extraction(SPE-GC-MS). Methods SVOCs in 1.0 L drinking water sample were enriched and purified by HLB solid phase extraction column, eluted by 2 ml ethyl acetate and 4 ml dichloromethane, purged and concentrated to 0.5 ml and volumed to 1.0 ml by ethyl acetate, separated by HP-5 ms ultra-inert capillary column(30 m×250 μm,0.25 μm), and then analyzed by selective ion scanning mode (SIM) coupled with GC-MS. Qualitative analysis was performed by retention time and characteristic ions, and quantitative analysis was performed by the internal standard method. Results Forty-three compounds of SVOCs were effectively separated and showed good linear relationship (r²≥0.995). The detection limits and quantitative limits of the method were 0.000 13-0.008 2 μg/L and 0.000 42-0.027 μg/L. The recoveries and precisions were 70.0%-138% and 0.3%-12.1%. Sixteen samples of different water were determined by the method, and ten compounds of SVOCs such as hexachlorobenzene, pentachloronitrobenzene and naphthalene were detected. Conclusion The method was sensitive and efficient, it was suitable for simultaneous detection of SVOCs in drinking water and provide a basis for public health emergencies.