Objective To investigate the antibiotic sensitivity and molecular typing characteristics of enteropathogenic Escherichia coli (EPEC) isolates in out-patient diarrhea cases in Beijing. Methods 461 strains were tested for drug susceptibility. Random sampling principle was adopted for strains isolated at different time in and counties, and 140 strains were characterized by pulsed field gel electrophoresis (PFGE). Results The total drug resistance rate of 461 EPEC strains was 84.8%(391/461). Ampicillin, tetracycline, and methylbenzidine/sulfamethoxazole had higher drug tolerance rates of 66.4% (294/443), 54.0% (249/461) and 45.6% (210/461), respectively. 461 strains were divided into 200 resistance spectrums. 54.0% (249/461) of the isolates were resistant to 3 or more kinds of antibiotics, and 1 strain was resistant to 12 antibiotics. Common resistance spectrum was only resistant to nalidixic, accounting for 4.3%(20/461). Ciprofloxacin, nalidixic, tetracycline, imipenem resistance rates and types of drug resistance spectrum were slowly increasing year by year. 140 strains produced 136 PFGE patterns, and there was no dominant pattern. The similarity coefficient was 53.3% to 100.0%. Conclusion The resistance of EPEC isolated from diarrhea patients in Beijing was serious, the spectrum of resistance was complex, and the variety of multiple resistant strains and resistance spectrum increased yearly. PFGE patterns showed diversity.