Objective To understand the prevalence, serotypes, phylogenetic characterization, and antimicrobial resistance of Vibrio parahaemolyticus in shellfish products sold in Haikou City. Methods Five types of seafood(sea white, sea discharge, blood snail, razor clam and mango snail)collected from 2014 to 2016 were tested for Vibrio parahaemolyticus according to China national standard GB 4789.7-2013 and the National Food Contamination and Hazardous Factor Risk Monitoring Manual. All isolates were further identified and serotyped. Virulence genes were also detected by real-time fluorescent polymerase chain reaction(PCR). Drug resistance of Vibrio parahaemolyticus to a panel of antimicrobial agents were detected by K-B method. Results Vibrio parahaemolyticus was observed in 65 out of 157 samples covered the five kinds of shellfish. The detection rate of white shellfish was the highest(63.6%,21/33). Among the 65 of Vibrio parahaemolyticus positive samples, O3 and O5 were the main serotypes. 26 strains were completely typed and the overall typing rate was 40.0%. The dominant serotype was O1∶K25. Results of resistance analysis showed that 65 isolated strains were generally resistant to ampicillin (95.4%, 62/65). The intermediate resistance rate of cefotaxime was high(33.8%, 22/65).65 strains of Vibrio parahaemolyticus produced 5 kinds of resistance spectrum to 8 kinds of antibiotics. The result of Kanagawa experiment showed that 65 strains were not hemolytic. No thermostable direct hemolysin(TDH)and thermostable related hemolysin(TRH)genes were detected in all 65 strains of Vibrio parahaemolyticus. Conclusion The pollution of Vibrio parahaemolyticus in shellfish sold in Haikou City was serious. The monitoring of seawater quality in the culture area should be strengthened to prevent Vibrio parahaemolyticus infection.