Objective To investigate the serotype and biochemical analysis of Salmonella isolates from food contaminant monitoring, food poisoning cases and isolates of employees' physical examination in Haizhu District of Guangzhou City from 2012 to 2018. Methods 416 strains of Salmonella were isolated from 2012 to 2018, including 28 food poisoning strains, 5 food contaminant monitoring strains, and 383 health practitioner strains, which were biochemically identified and serotyped. Results There were 40 serotypes identified from 416 strains of Salmonella, including 56 S.Typhimurium strains (13.5%), 46 S.Enteritidis strains (11.1%), 41 S.Derby strains (9.9%), and 273 other serotypes (65.6%). Serotypes in group A-F were the most common in the region, and many rare serotypes were detected. Conclusion Community and serotypes of Salmonella in this region were numerous and widely distributed in the population. S.Typhimurium, S.Enteritidis and S.Derby were the dominant serotypes in the region. The screening of Salmonella serotypes was of great significance to the diseases caused by the bacteria.