Objective To investigate and trace the Vibrio parahaemolyticus food poisoning at a hotel dinner, providing reference for the study of similar food poisoning. Methods Epidemiological and food hygiene investigation and pulsed field gel electrophoresis(PFGE)technology were used to investigate and analyze the incident. Results Twenty-six cases of food poisoning were confirmed, and the incidence of poisoning was 3.7% (26/710). Thirteen Vibrio parahaemolyticus were detected in 26 cases and 16 chefs in the hotel. The serotypes were O3∶K6, and the PFGE fingerprints were the same. Conclusion The food poisoning caused by Vibrio parahaemolyticus was identified by comprehensive analysis of epidemiological and food hygiene investigation and laboratory test result. Food safety supervision departments should strengthen the supervision and management of catering enterprises and prevent the recurrence of such accidents.