Objective For implementation of labeling regulations, a construct-specific real-time polymerase chain reaction (PCR) method for quantitative detection of ArcticTM apple was established in this study. Methods Primers and TaqMan probe were designed based on the specific sequence of ArcticTM apple. The specificity, sensitivity and repeatability of the developed method were examined, respectively. Results The specificity test of this method showed it specific to ArcticTM apple; The limit of quanification was 20 copies and the amplification efficiency was 96%. It had good repeatability. Conclusion This construct-specific real-time PCR method was suitable for the identification of ArcticTM apple.