Objective To study the effects of Chrysanthemum ethanol extract (CME) on rat offsprings with continuously exposure during parental, intrauterine, lactation and post-weaning. Methods CME was mixed into the feed to feed rats, which were divided into control, low, medium and high dose groups with dosage of 0,1, 3,9 g/kg BW. Extended one-generation was carried out according to OECD TG 443 with 20 pregnant rats and 40 offspring in each group (half female and half male). CME was continuously given to offspring rats for 13 weeks after weaning. During the experiment, growth, reproductive development, hematology, biochemistry and histopathology were measured. Results The eyes opening time of the infant rat in the middle and high dose group of CME was significantly later than that of the control group. The weight of female offspring rats from postnatal day(PND)56-112 was significantly higher than that of the control group. In the high-dose group and low-dose group of female offspring rats, red blood cell(RBC) and hemoglobin (HGB) were decreased. In the high-dose group of female offspring rats, glutamic-pyruvic transaminase(ALT)increased. In the high-dose group of female rats, glutamic oxalacetic transaminase(AST) and serum phosphorus(P) increased. The glucose (GLU) decreased and the lactate dehydrogenase (LDH) increased, in CME treated female offspring group. The potassium(K⁺) decreased in males offspring rats of high and low dose groups. The spleen coefficient in the high dose group was significantly higher than that in the control group. The above differences were statistically significant (F=2.818-42.75, P<0.05). Conclusion Low dosage CME promotes the body growth, the RBC, HGB and LDH were affected in female offspring rats; middle and high dosage CME affects development, liver function of infant rat,high dosage affects spleen coefficient in male and female offspring rats.