Objective To understand the contamination of 4 alternaria toxins in wheat flour sold in Henan Province in 2016-2017. Methods Totally 182 wheat flour samples were collected from 9 cities in the main grain producing areas of Henan Province from 2016 to 2017.The samples were detected with national handbook for monitoring food contamination and hazardous factors.The data were analyzed by chi-square test and correlation analysis. Results Among the 182 samples of wheat flour in 9 regions, 4 kinds of alternaria toxins were all detected.The detection rate of alternariol (AOH) was 10.4%(19/182)with concentration range from 0.50 to 14.21 μg/kg; the detection rate of alternariol methyl ether (AME) was 42.9%(78/182) with concentration range from 0.05 to 38.73 μg/kg; the detection rate of tenuazonic acid (TeA) was 91.2%(166/182) with concentration range from 0.50 to 134.23 μg/kg and the detection rate of tentoxin (TEN) was 45.6%(83/182) with concentration range from 0.05 to 17.42 μg/kg. Conclusion The contamination of the alternaria toxins of wheat flour in the circulation link (farm trade market and store) was exit in Henan Province, but the level of contaminationwas lower than reported in the literature.