Microorganisms are widely used in food industry during the production process of food and food additives.The safety of the strains are directly related to food safety and public health. Pathogenicity is one of the important evaluation indicators for the safety of the strains. It is also required in the application of novel food and new food additives related to microorganisms in China, but there is no standard method for safety evaluation so far.This paper reviews the regulations in pathogenicity evaluation of strains from international organizations and other countries in food, feed, etc. which will provide useful informations for the development of appropriate safety evaluation method in China.