Objective To develop a method for Vibrio vulnificus(V.vulnificus)detection from aquatic products for the contamination monitoring. Methods The selective medium specific for V.vulnificus detection was optimized by inoculating 18 V.vulnificus strains and common competing flora in aquatic products into 8 selective enrichment broth and 4 selective plates. Then the method was established and validated which was combined polymerase chain reaction(PCR) method with enrichment culture method for detecting V.vulnificus through comparing the performance of these two method. And the method was applied to V.vulnificus detection from Beijing commercial aquatic products for the contamination monitoring. Results The modified selective enrichment broth was applied to V.vulnificus detection. The isolation rate in Beijing retail aquatic products was 15.7%(18/115).Isolation rate in shellfish products was 23.4%(15/64). Isolation rate in crab products was 8.3%(1/12). Isolation rate in fish products was 5.3%(1/19). Isolation rate in shrimp products was 5.0%(1/20). Conclusion The method that combined PCR with traditional enrichment culture method exhibited a better isolation rate. A higher contamination rate of V.vulnificus in shellfish products was found which should be paid attention to.