Objective To study the etiological characteristics of Yersinia enterocolitica (Ye)isolated from diarrheal patients and household refrigerators, and to provide basis for scientific prevention and control of Ye. Methods Stool samples were collected from diarrhea patients from two outpatients in two hospitals in Shunyi District, Beijing in 2017.Smear samples were collected from 83 household refrigerators in Shunyi District.Ye was isolated and cultured, and the isolated stains of Ye were tested for toxin genes,pulsed field gel electrophoresis (PFGE) molecular typing and drug resistance tests. Results The positive rate of Ye was 0.27% (1/372) in stool samples of diarrhea patients and 6.02% (5/83) in household refrigerators. The strains isolated from refrigerators only carried the ystB gene and the strains isolated from diarrhea patients carried ail,ystA, virF and yadA genes. Six Ye strains showed 4 PFGE patterns with a low similarity.All Ye strains were resistant to ampicillin, amoxicillin/ clavulanic acid and cefazolin,and the Ye strain isolated from diarrhea patient was resistant to nalidixic acid. Conclusion The prevalence of Ye was not high in the patients of diarrhea in Shunyi District of Beijing.Ye contaminationin household refrigerators was more serious.Ye pathogenic characteristics in environment and diarrhea patient were different.