Objective The purpose of this research was to isolate and identify the pathogenic bacterium which might cause the bacterial food poisoning accident in 2016 in Putian. The traceability analysis of the pathogenic bacterium was also emphatically investigated. Methods The bacteria in the collected samples were isolated, cultured, biochemical identified and serotyped. Pulsed field gel electrophoresis(PFGE)were used for the homology analysis of the isolated strains. Virulence gene detection and drug susceptibility analysis of the isolated strains were also applied. Results A total of 23 strains of Salmonella enteritidis were isolated, of which 8 strains were isolated from the retained food samples, 2 strains from the cooks and 13 strains from the patients. The result of cluster analysis using BioNumerics indicated that all of the strains were 100% homological. The virulence gene invA were detected in all these strains with the same drug-resistant spectrum. Conclusion This food poisoning accident was caused by Salmonella enteritidis which carried by the cooks to the food during the preparation of the cold cuts and the fresh fruit platter. PFGE were useful for the traceability analysis of bacterial caused food poisoning.