Objective To understand the distribution characteristics of Vibrio parahaemolyticus (VP) in aquatic products and their environment samples in Huzhou, then to explore possible influencing factors of VP pullution in freshwater products. Methods One thousand three hundred and eighty-eight aquatic products and their environment samples were collected in Huzhou between 2014 to 2017. Aquatic products and environment samples were tested for VP, O group of serum and the virulence genes tlh, tdh and trh, respectively. To the distribution characteristics of VP in freshwater products, seafoods and environment samples was analyzed and compared. The possible influencing factors of VP pollution in freshwater products was further analyzed in Huzhou City. Results The rate of VP in aquatic products was 25.68%(265/1 032), of which freshwater products and seafood were 30.33% (175/577) and 19.78% (90/455) respectively, and the difference was statistically significant (χ²=14.83, P<0.001). The rates of VP in subsoil sediment and water body from sales links were 0.86% (1/116) and 13.71% (17/124) respectively. There is no VP detected in the water body from breeding links. By multivariate logistic regression analysis, the aquatic species, preservation method, sales links and time affected VP pollution of freshwater products to a certain extent. There was no significant difference in the distribution of virulence genotypes between aquatic products and environmental samples (P＞0.05), all of which were dominated by tlh+tdh-trh-. In the serotype distribution, there was no significant difference between freshwater products and seafood (P＞0.05), which O3 were mainly serotypes, followed by O4 serotypes. Conclusion Freshwater products was seriously polluted by VP in Huzhou, and pollution mainly comes from cross-contamination in the sales links. Monitoring and management should be further strengthened.