Objective To establish a method for the determination of 5 arsenic species including arsenite [As (Ⅲ)], arsenate[As (Ⅴ)], monmethyl arsenic (MMA), dimethyl arsenic(DMA) and arsenobetaine (AsB) in the soaking solution of healthy food teabag by high performance liquid chromatography-inductively coupled plasma mass spectrometry method (HPLC-ICP/MS). Methods The samples were extracted by hot extraction. Five arsenic species were separated in 10 min by Dionex IonPac AS19 (4 mm×250 mm, 10 μm) anion exchange column with 30 mmol/L ammonium carbonate (pH=9.5) as the mobile phase for isometric elution. Results The linear ranges for 5 arsenic species were between 0.2-300 μg/L with the linear coefficients more than 0.999, and the detection limits were between 0.04-0.08 μg/L. The repeatability of the method was examined by precision. The relative standard deviation (RSD) for the 5 arsenic species were less than 5% and the recoveries were between 80.3%-121.1%. Conclusion The method was suitable for the analysis and determination of arsenic species in the soaking solution of healthy food teabag. The experiment proved that the main arsenic species in healthy food teabag were As (Ⅲ) and As (Ⅴ). Attention should be paid to the risk of inorganic arsenic.