Objective To study and compare the separation effect of four types of selective culture media for Pseudomonas cocovenenans subsp. farinofermentans, including potato dextrose agar (PDA), modified potato dextrose agar (mPDA), Pseudomonas cocovenenans subsp. farinofermentans agar (PCFA) and tremella yolk chloramphenicol agar (TYCA), and provide technical support for the national standard GB/T 4789.29-2003. Methods Pseudomonas cocovenenans subsp. farinofermentans was inoculated on four types of media, the colony morphology and the growth rate were observed. Specificity test of growth status was carried out on four types of media by inoculating ten types of pathogenic bacteria and common environmental bacteria. The separation effect of four types of media was verified in different standard strain-added samples, including stool food and soil, by directly coating and enrichment streaking. Results The growth of 8 and 6 pathogenic bacteria could be inhibited respectively on mPDA and PCFA, and the growth rate of Pseudomonas cocovenenans subsp. farinofermentans could reach above 75%.The target bacteria in mPDA and PCFA had showed apparent difference compared with most of the interfering bacteria; while the difference of their colonies was not significant in PDA medium and TYCA. In the standard strain-added experiments, both directly coating and enrichment streaking of different samples such as stool, soil and food, the detection rate of mPDA and PCFA was above 80%,and was significantly higher than that of PDA and TYCA. Conclusion The separation effect of mPDA and PCFA was significantly better than that of PDA, and these two selective media could be added to the revised standard.