Objective This research explored the spatial distribution and spatial auto-correlation of cadmium content level in crayfish in Qianjiang, Hubei, which aimed to provide basis for optimizing the local crayfish breeding industry. Methods The detection data of 760 samples at 38 sample points were analyzed by the spatial visualization and auto-correlation. Results The crayfish with relative high level of cadmium source from the northwest and south part of Qianjiang, especially guanghuasi in the northwest. The Moran's I was -0.04, and the Z score was -0.10 and the P value was 0.92 (P>0.05). Conclusion The cadmium content in crayfish in Qianjiang didn't present spatial clustering. And the level was lower than the maximal residual level which indicate a favorable breeding environment in Qianjiang.