Objective The aim of this study was to derive the health-based guidance values for nonylphenol (NP), which would lay the foundation for further evaluating the human health risk of nonylphenol. Methods According to the principles and method ology of risk assessment and systematic review, using a weight of evidence approach, high-quality studies was selected into database by systematically reviewing of toxicology data and uncertainty factors. Results After comprehensive assessment, the toxicity information is provided by a high quality multi-generation reproductive toxicity study and lowest observable adverse effect level (LOAEL) method was choen. Renal toxicity was selected as the critical effect, and a LOAEL was considered as the point of departure for extrapolation. Conclusion The tolerable daily intake value of 0.025 mg/kg BW was recommended as the final health-based guidance value for NP.