Objective To establish a method for the determination of 22 sulfonamides in livestock products by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Samples were extracted by acetonitrile and the fat was removed by hexane. Then the extract was purified by cation exchange solid phase extraction. The analytes were separated on a Waters Acquity UPLC BEH C₁₈ column (2.1 mm×100 mm, 1.7 μm) and detected by positive electrospray ionization in multiple reactions monitoring (MRM). Results The method showed a good linearity within a certain concentration ranges with relative coefficient r>0.997. The detection limits of 22 sulfonamides in porcine muscle, liver, and kidney were 0.03-0.3 μg/kg, and the quantification limits were 0.1-1 μg/kg. The average recoveries of 22 sulfonamides at the spiking levels of 1,2 and 5 μg/kg were in the range of 63.0%-115.6%, with the relative standard deviations in the range of 1.2%-15.9%. Conclusion The method was rapid, simple and accurate for the simultaneous determination of 22 sulfonamides in livestock products.