Objective To evaluate the genotoxicity of Coreopsis tinctoria(C.tinctoria) Nutt.extract and provide scientific basis for its edible safety. Methods The test subject was a concentrated solution of C.tinctoria Nutt., equivalent to 1 g of dry matter per milliliter. 0.008,0.04,0.2,1 and 5 mg C.tinctoria Nutt.extract groups were set in Ames test,and the number of reversional mutation colony was counted directly in culture medium.C.tinctoria Nutt.extract 1.25,2.5 and 5 mg/ml groups were set in chromosome aberration test of mammalian cells in vitro, chromosome aberration cell number was directly counted. C.tinctoria Nutt.extract 5,0 and 20 g/kg bodyweight groups were set in micronucleus test, and sperm abnormality test and chromosome aberration test of spermatogonial cell or spermatocyte in mice. 1 000 polychromatic erythrocytes (PCE),1 000 complete sperms and 100 metakinesis cells per animal were observed by microscopy,PCE count with micronuclei count, type of malformed sperms and chromosome aberration cell number were recorded. Results The result of genetic toxicity test were negative. Conclusion C.tinctoria Nutt. extract did not cause any measurable genotoxicity.