Study on the toxicity of four kinds of Alternaria toxins on human esophageal epithelial cell Het-1 A in vitro
CSTR:
Author:
Affiliation:

(Key Laboratory of Food Safety Risk Assessment of Ministry of Health,China National Center for Food Safety Risk Assessment,Beijing 100021,China)

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To study the acute cytotoxicity of four kinds of Alternaria toxins alternaiol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA) and tentoxin (TEN) on human esophageal epithelium cell Het-1 A in vitro. Methods Het-1 A was treated with different concentrations of 4 kinds of Alternaria toxins. The effects of 4 kinds of Alternaria toxins on cell proliferations, cell apoptosis, cell cycle distribution and cysteinyl aspartate specific proteinase-3 (capase-3) activity were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT), annexin V-fluorescein isothiocyanate/propidium iodide(Annexin V-FITC/PI)double staining method, PI single staining method and spectrophotometry, respectively. Results The half maximal inhibitory concentration (IC50) of AOH, AME, TeA and TEN on Het-1 A was 54.31,3.38,1.91 and 141.96 μmol/L, respectively. The 4 kinds of Alternaria toxins could induce the apoptosis of Het-1 A and also could affect the cell cycle distribution through increasing the percent of G2-M phase. AOH and AME could enhance the activity of caspase-3 in a positive dose-dependent manner to induce the apoptosis of Het-1 A. Conclusion AOH, AME, TeA and TEN could cause acute cytotoxicity on Het-1 A through inhibiting cell proliferation, inducing cell apoptosis and G2-M cell cycle arrest etc.

    Reference
    Related
    Cited by
Get Citation

HAN Xiao-min, HAN Chun-hui, LI Feng-qin. Study on the toxicity of four kinds of Alternaria toxins on human esophageal epithelial cell Het-1 A in vitro[J].中国食品卫生杂志,2018,30(1):1-5.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:September 15,2017
  • Revised:
  • Adopted:
  • Online: April 02,2018
  • Published:
Article QR Code