Objective To investigate the frequency of nutrition fortifier compounds in pre-packaged foods, and to provide reference for the revision of food fortifier standard and its quality standards in China. Methods Cross-sectional survey was performed between April to October in 2016, according to the food classification system of National Food Safety Standard for the Use of Food Nutritional Fortifier in Foods (GB 14880-2012). A total of 3 760 samples of pre-packaged foods were collected, special dietary foods, salt and salt products were not included. The sources of the compounds and the frequency of the compounds was statistically analyzed. Results A total of 489 fortified foods were separated from 3 760 survey samples, and the cumulative frequency of these compounds were 2 689 times. These 133 fortifier compounds in appendix B of GB 14880-2012 and its subsequent amendments, 52.6% (70/133) of them were used more than once, among which the frequency of calcium and zinc were more than 200 times. In additional, there were 47.4% (63/133) of the compounds were not used, further analysis showed that 47.6% (30/63) of them didn't have the related quality standards. The possible reasons were the cost, storage difficulties and low bioavailability, etc. Conclusion The application of nutrition compounds and its quality standard status were elucidated by this study, and it provided useful information for the revision of GB 14880-2012. For the substance without quality standards, it should be updated as soon as possible. The present compounds list should be reevaluated during GB 14880-2012 revision.