Objective To understand the situation of mycotoxin residues in puerh tea from Guangzhou City, and provide reference basis for risk assessment and government supervision. Methods A total of 432 puerh tea samples were collected from wholesale markets, retail stores, super markets, catering units, online sellers and tea fair in Guangzhou City from 2013-2015. The concentrations of aflatoxin B₁, B₂, G₁, G₂ in the samples were determined by purification column high performance liquid chromatography (HPLC) with post column derivatization method. Nivalenol, deoxynivalenol and zearalenone were determined by high performance liquid chromatography-mass spectrometry (HPLC-MS). Results No aflatoxin B₁, B₂, G₁, G₂, nivalenol, deoxynivalenol and zearalenone in puerh tea samples could be detected by HPLC and HPLC-MS method. Conclusion There was no mycotoxin detected by HPLC and HPLC-MS national standard method. Puerh tea in the markets of Guangzhou City was not contaminated with mycotoxins.