Objective A method was developed for simultaneous quantification of eight E vitamers (tocopherols and tocotrienols with α, β, γ and δ type) in nut matrix by hydrophilic interaction liquid chromatography (HILIC) after pretreating with accelerated solvent extraction. Methods Nut samples were extracted using accelerated solvent extraction (ASE), the separation of 8 anlaytes was achieved using BEH amide amino column (150 mm×3.0 mm, 1.7 μm) and the mobile phase was 90% hexane and 10% methyl tert-butyl ether-tetrahydrofuran-methanol (20∶0.1, V/V). Results The detection limits were ranged from 0.032 to 0.070 mg/kg with linear correlation coefficient above 0.998, while quantification limits were 0.096-0.210 mg/kg. Intraday and interday recoveries of spiking standards were 87.5%-114.2%, and relative standard deviations (RSDs) were all less than 12%. Conclusion The established method could be applied in the simultaneous detection of vitamin E isomers in nuts due to its excellent sensitivity, recovery and reproducibility.