Objective A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for determination of beauvercin (BEA), enniatin A (ENA), enniatin A₁ (ENA₁), enniatin B (ENB) and enniatin B₁ (ENB₁) in corn, wheat and their products was developed. Methods Samples were extracted with acetonitrile-water (85∶15, V/V), cleaned up with solid phase extraction column followed by HPLC-MS/MS determination with electrospray positive ionization (ESI+) under multiple reaction monitoring (MRM) mode, and with 2 mmol/L ammonium acetate water-acetonitrile as the mobile phase. Results The limits of detection for BEA and 4 kinds of enniatins (ENNs) ranged from 0.01 to 0.12 μg/kg for corn and corn products and 0.02 to 0.21 μg/kg for wheat and wheat products, and the limits of quantification ranged from 0.02 to 0.44 μg/kg and 0.05 to 0.68 μg/kg for both cereals. The mean recoveries ranged from 91.6% to 149.7% for corn and corn products and 100.5% to 128.2% for wheat and wheat products, respectively. The matrix induced suppression or enhancement effect ranged from 88.0% to 101.5% and 55.8% to 106.5% for both cereals, respectively. The method for BEA and ENNs determination showed good linearity and precision in all matrixes with correlation coefficient above 0.99, and the relative standard deviations were less than 15%. Conclusion The method developed was of simple operation, high sensitivity and good accuracy, which could be used for BEA and 4 kinds of ENNs determination in corn and wheat and their products.